Seasonally frozen soil covers the whole area of Northwestern China where earthquakes have happened frequently in recent years. In order to study the seismic response characteristics of seasonally frozen soil sites in Northwestern China, shaking table tests of seasonally frozen soil sites were carried out, and three-dimensional solid finite difference models with different freezing depths were established. Seismic responses and soil dynamic shear stress-strain relationship under different seismic excitations were analyzed. Results showed that the existence of the frozen soil layer effectively restrained the ground motion energy, while the horizontal displacement of the site in frozen status was larger than that in unfrozen status. Besides, the vertical displacement showed a significant layered seismic settlement. The peak amplification coefficient of the seasonally frozen soil first increased, then decreased and finally increased again. It was found that the thicker the frozen layer, the smaller the peak amplification coefficient. In addition, the dynamic shear stress-strain relationship of soil exhibited obvious nonlinearity, and the frozen soil layer can dissipate the seismic energy during earthquakes.