A mathematical wind field model for downburst is introduced and applied to calculate the distributions of wind loads on transmission lines. The wind field and structural parameters analysis for the downburst and multi-spanned transmission lines are performed to investigate the critical cases of the conductor wind load acting on the tower using the nonlinear finite element method. The result shows that:(1) the maximum lateral load value of the tangent tower is the same as that of the tension tower, however, the maximum longitudinal load value of the latter is significantly larger than that of the former;(2) The maximum lateral and longitudinal conductor wind loads of the tangent tower appear at the end and sub-end of the eight-span transmission line, while the maximum longitudinal loads of the tension tower appears in the four-span transmission line;(3) The maximum conductor wind loads are affected by the downburst diameter, the relative position between the center of downburst and the target tower, as well as the number of spans. Therefore, there exist specific most unfavorable cases for both lateral and longitudinal conductor wind loads on tangent or tension towers. By summarizing the above parameter analysis, a set of normalized wind field parameters for three critical cases of most unfavorable transmission line wind loads are proposed, which will provide an important analytical basis for the reasonable determination of the design value of the conductor wind loads under downburst.