In order to study the seismic response characteristics of the buried pipeline in soil with soft interlayer，the software ADINA and nonlinear constitutive model was adopted in this paper. The interaction model was also utilized to simulate the contact of pipeline and soil. The influence of different factors including the buried depth, the soft interlayer thickness and the dip angle on seismic response of pipeline was obtained. Results show that the influence of the soft interlayer indexes on the seismic dynamic response of buried pipeline is different. The soft interlayer has an effect of isolation with a proper thickness and a suitable buried depth. When enhancing the inhomogeneity of the ground，soft interlayer will have unfavorable impact on the seismic response of the buried pipeline. The effective stress and deformation of the pipeline decrease with the increase of the soft interlayer thickness. The soft interlayer plays a certain buffering role when its thickness reaches a certain value. The effective stress and deformation of the pipeline decrease with the increase of the buried depth of the soft interlayer，but the influence degree is less than that of the interlayer thickness. The effective stress and deformation of the pipeline increase with the increase of the dip angle of the soft interlayer which increases the inhomogeneity of the ground.